For LC/MS, we need a robust and reproducible sample preparation

Porvair Sciences published an in-depth review of how their Microluter(r) Reverse Phase Solid Phase Extraction microplates are a reliable and reproducible SPE LC/MS sample preparation technique for a variety of acidic, neutral, and basic analytes.

Microlute hybrid Solid Phase Extraction technology. Image credit: Porvair Science

Solid Phase Extraction is widely acknowledged as a preferred method to prepare samples. It can be used to clean samples before they are analysed using hyphenated techniques such as GC/MS or LC/MS. The analyst will be able to see many advantages to Solid Phase Extraction, including less downtime and troubleshooting, cleaner and more reproducible chromatograms, less contamination from substances, and greater the rate of recovery of analytes. Traditional SPE methods employ loose-filled resins, which could result in problems like voids in the sorbent bed that can cause channeling and inconsistency flow-through. These undesirable effects cause reduced interactions between analytes and the active resin, resulting in inconsistent results and poor recovery of the analytes.

Acidic analyte recover – percent RSD comparison Microlute CP with competitor SPE products. Image credit: Porvair Sciences

These microplates, Microlute(r), microplates for CP SPE from Porvair Sciences, were designed to address these issues. They have a distinctive polymer structure, which is a network of pores evenly distributed and retentive. This hybrid technology ensures even liquid flow rates throughout the SPE procedure, which provides enough time for Van der Waals forces of interaction to take place between the solid phase sorbent and the analytes. This results in an extremely reproducible method for SPE.

The application report contains experimental data that show how Microlute(r) and microplates containing CP RP 96 wells selectively retain, elute an array of basic, neutral, or acidic compounds. The results show that Microlute(r), CP’s percentage relative standard deviation values are significantly lower than commercial SPE plates. This leads to more reproducible results. The application note also indicates that there is no loss of analytes during the loading step of the SPE process, resulting in high rates of recovery for all types. The Microlute(r) CP RP 96 well-plate for solid phase extractions has also shown to offer significant advantages for the recovery of basic analytes that are hydrophobic.

Microlute CP products. Image credit: Porvair Sciences

To read the application report in full please visit For further information on Microlute(r) CP Reverse Phase Solid Phase Extraction (SPE) microplates please visit or contact Porvair Sciences at [email protected] or call +44 1978 666222 / +1 800 552 3696.

Porvair Sciences is a global producer of consumables and tools for life science and analytical workflows. It also includes JG Finneran, Porvair Kbiosytems, and Porvair Sciences. The company offers a broad assortment of products, including microplate technologies, glass vials and automated laboratory equipment. It is dedicated to providing top-quality products that will enhance productivity and analysis, which will allow scientists to speed up their scientific discovery.

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Gemma Wilson

Gemma is a journalism graduate with keen interest in covering business news – specifically startups. She has as a keen eye for technologies and has predicted quite a few successful startups over the last couple of years.

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